منابع مشابه
OpenComet: An automated tool for comet assay image analysis
Reactive species such as free radicals are constantly generated in vivo and DNA is the most important target of oxidative stress. Oxidative DNA damage is used as a predictive biomarker to monitor the risk of development of many diseases. The comet assay is widely used for measuring oxidative DNA damage at a single cell level. The analysis of comet assay output images, however, poses considerabl...
متن کاملStatistical analysis of comet assay results
The comet assay can distinguish small differences in DNA damage between different samples of cells, implying that statistical tests are important to assess whether this occurs by chance. Excellent scholarly papers with concise descriptions of statistical analysis and recommendations for tests have been published (Lovell et al., 1999; Lovell and Omori, 2008). We often come across publications th...
متن کاملAutomated Comet Assay Imaging and Dual-Mask Analysis to Determine DNA Damage on an Individual Comet Basis
Mammalian cells incur DNA damage and alterations as a result of internal factors, such as stress, environment, oxidation and metabolism, as well as exposure to external stimuli, such as chemical compounds, UV and cosmic radiation. Genotoxicity studies aim to identify and understand the mechanisms of DNA damage and repair, and also to seek methods of pathway regulation and dysregulation for impr...
متن کاملAutomated Imaging and Analysis of a Novel Comet Assay to Enable High Throughput Genotoxicity Testing
Human contact to genotoxins is inevitable, as agents detrimental to our DNA are present in our environment, and also a result of our own cellular metabolism where they can contribute to cell death, gene mutations, and even cancer, disease and aging1,2,3,4. At the same time, therapeutic approaches such as chemotherapy and radiation treatment rely on initiation of tumor cell genotoxicity to produ...
متن کاملThe Comet Assay: Automated Imaging Methods for Improved Analysis and Reproducibility
Sources of variability in the comet assay include variations in the protocol used to process the cells, the microscope imaging system and the software used in the computerized analysis of the images. Here we focus on the effect of variations in the microscope imaging system and software analysis using fixed preparations of cells and a single cell processing protocol. To determine the effect of ...
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ژورنال
عنوان ژورنال: Cytometry
سال: 1999
ISSN: 0196-4763,1097-0320
DOI: 10.1002/(sici)1097-0320(19990201)35:2<134::aid-cyto5>3.0.co;2-9